Caspase 3 antibody (AA 55-160)

Catalog No. ABIN6989111

The Rabbit Polyclonal anti-Caspase 3 antibody has been validated for WB, ELISA, IF, IHC (p) and IHC (fro). It is suitable to detect Caspase 3 in samples from Human and Rat.

Quick Overview for Caspase 3 antibody (AA 55-160) (ABIN6989111)

Target: Caspase 3 (CASP3)

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Caspase 3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Caspase 3 Antibody

Binding Specificity: AA 55-160

Cross-Reactivity: Human, Rat

Predicted Reactivity: Mouse

Purification: Purified by Protein A.

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 55-160 of human Caspase-3 (NP_004337.2).

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF()

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for Caspase 3

Target: Caspase 3 (CASP3)

Alternative Name: Caspase-3

Background:

Synonyms: CPP32,CPP32B,SCA-1,Active Caspase 3,CASP3,active Caspase-3,Caspase 3,Caspase-3 p12,caspase-3

Background: This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein.

Gene ID: 836

UniProt: P42574

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Sensory Perception of Sound, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation